coffee, tea, chocolate and mushrooms: A mismatch of mediums?

The flavor of mushrooms is not for everyone, and many ‘functional’ food companies are well aware of this. Some of these companies formulate mushrooms in teas, chocolates and coffee so that their consumers can benefit from the mushrooms without having to deal with their ‘mushroomy’ flavor. In fact, many of them claim that mushrooms in these formulations even have enhanced medicinal properties. Although I am all for getting more mushroom medicine into more people, I think it is important for people to look into the interactions between different biomolecules before making claims about the medicinal quality of their products.

The interactions between various polyphenols (like tannins), and polysaccharides, are of particular interest. Polyphenols are found in varying degrees in coffee, tea and chocolate, and are also found in many other herbs and foods, but for the purposes of this article I will keep our discussion specific to foods that mushrooms are often formulated with (1, 2).

The polyphenols present in coffee, tea and chocolate have many health benefits, including anti-oxidant, anti-inflammatory and anti-microbial activity (3). The issue is not the medicinal qualities of polyphenols, but that when blended with mushroom extracts, they create bonds with polysaccharides (4, 5).

Abstract Image
Polysaccharides (left) Polyphenols (center) Polysaccharide-Polyphenol Complex (right)
J. Agric. Food Chem. 2013, 61, 19, 4533–4538
Publication Date:April 26, 2013

Immune-modulating polysaccharides are the most well-researched component of mushroom medicine. In order for polysaccharides to exert an immune-modulating effect, they need to bind to specific receptors on immune cells within the gut-associated-lymphoid-tissue (GALT) (6). If the polysaccharides are bound to polyphenols, they cannot bind to these receptors, and the immune-modulating benefits that these products are touted for become obsolete.

Important caveats:

The bonds between polysaccharides and polyphenols are hydrogen bonds, and are easily affected by temperature and pH:

Temperature: Bonds become less stable as temperature increases from 68F to 104F. You may want to blend your mushroom extracts with your coffee, chocolate and tea while the temperature is high. If the temperature is too low, the bonds will remain strong, even in stomach acid.

pH: Bonds become less stable as pH increases from 2 to 9. Stronger bonds seem to be made under more acidic conditions. The pH of stomach acid is around 1.5 to 3.5. Therefore, stomach acid will NOT break these bonds, and they will remain intact as the complexes enter the small intestine. Polysaccharides contained in these complexes will NOT be available to bind immune receptors in the GALT, and their immune-modulating properties will be nullified.

But all is not lost!

Polyphenols as well as polysaccharides exert many benefits on gut microbiota, and a healthy gut microbiome is imperative to strong immunity (7, 8, 9). I suspect that if these polyphenol-polysaccharide complexes make their way to the colon, their beneficial impacts on the microbiome do enhance the immune system, but through a different pathway than in the small intestine.

Work Cited

  1. Savolainen H. Tannin content of tea and coffee. J Appl Toxicol. 1992 Jun;12(3):191-2. doi: 10.1002/jat.2550120307. PMID: 1629514.
  2. Jalil AM, Ismail A. Polyphenols in cocoa and cocoa products: is there a link between antioxidant properties and health?. Molecules. 2008;13(9):2190-2219. Published 2008 Sep 16. doi:10.3390/molecules13092190
  3. Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr. 1998 Aug;38(6):421-64. doi: 10.1080/10408699891274273. PMID: 9759559.
  4. Wang Y, Liu J, Chen F, Zhao G. Effects of molecular structure of polyphenols on their noncovalent interactions with oat β-glucan. J Agric Food Chem. 2013 May 15;61(19):4533-8. doi: 10.1021/jf400471u. Epub 2013 May 6. PMID: 23647238.
  5. Li, R., Zeng, Z., Fu, G., Wan, Y., Liu, C., & McClements, D. J. (2019). Formation and characterization of tannic acid/beta-glucan complexes: Influence of pH, ionic strength, and temperature. Food Research International120, 748–755.
  6. Camilli, G., Tabouret, G., & Quintin, J. (2018). The Complexity of Fungal β-Glucan in Health and Disease: Effects on the Mononuclear Phagocyte System. Frontiers in Immunology9, 673.
  7. Kumar Singh A, Cabral C, Kumar R, et al. Beneficial Effects of Dietary Polyphenols on Gut Microbiota and Strategies to Improve Delivery Efficiency. Nutrients. 2019;11(9):2216. Published 2019 Sep 13. doi:10.3390/nu11092216Ho Do M, Seo YS, Park HY. Polysaccharides: bowel health and gut microbiota. Crit Rev Food Sci Nutr. 2021;61(7):1212-1224. doi: 10.1080/10408398.2020.1755949. Epub 2020 Apr 22. PMID: 32319786.Savolainen H. Tannin content of tea and coffee. J Appl Toxicol. 1992 Jun;12(3):191-2. doi: 10.1002/jat.2550120307. PMID: 1629514.
  8. Ho Do M, Seo YS, Park HY. Polysaccharides: bowel health and gut microbiota. Crit Rev Food Sci Nutr. 2021;61(7):1212-1224. doi: 10.1080/10408398.2020.1755949. Epub 2020 Apr 22. PMID: 32319786.
  9. Zheng, D., Liwinski, T. & Elinav, E. Interaction between microbiota and immunity in health and disease. Cell Res 30, 492–506 (2020).

Medicinal Mushrooms – Going Viral

Mushrooms have been used as both food and medicine since antiquity. One of my favorite poems, discovered in an ancient Egyptian temple, illustrates this history: “Without leaves, without buds, without flowers, yet from fruit; as food, as tonic, as medicine: the entire creation is precious.” At a time when viral epidemics are inevitable and the current COVID-19 pandemic has presented in most of the world, antiviral therapies are possibly being investigated now more than ever before.  This paper explores the use of medicinal mushrooms as antivirals in in vivo (human and animal) and in vitro (petri dish) experiments and how these experiments may inform us on the utilization of these fungi as antiviral therapies.

Medicinal mushrooms are known as biological response modifiers. This physiologic modification is largely a result of the interaction of various mushroom constituents, primarily polysaccharides, with the immune system. Therefore, to understand the role that medicinal mushrooms play as antiviral agents, it is imperative to understand the happenings of the immune system in response to a viral pathogen and the interplay between mushrooms, their constituents, and this system. Unlike pharmaceutical antivirals, the actions of medicinal mushrooms are not straightforward, and there is no absolute rule that mushrooms stimulate or depress immunity. Mushrooms contain many constituents and are dynamic in their interplay with the human body.

Overview of anti-viral immunity 

The initial immune response to a new pathogen is facilitated by the innate immune system (innate meaning inborn or natural). This is our first response to non-self organisms, and requires no other stimulation than the pathogen itself. It is the response that is ready to go at all moments in the day and persistently protects the human body from infection. It is easy to imagine that an altered or defective innate immune response can have a detrimental effect on the ability to fight disease.

The innate immune response to a virus is multidimensional. There is a massive amount of cell-to-cell communication and different chemicals (called cytokines) are released to make this communication possible. Once an epithelial cell (cells that make up the surface of different body tissues like skin, lungs, etc) is infected with a virus, Type 1 Interferon (Interferon-α, a cytokine) is released and has three major functions: to induce resistance to viral replication in all cells, to increase expression of ligands for receptors on natural killer (NK) cells, and to activate NK cells to kill virus infected cells. NK cells are lymphocytes (a small white blood cell that is found primarily in the lymphatic system) that defend against viral infections and tumor cells via cytokine stimulation and direct killing of infected cells. NK cells provide such a vital role in antiviral immunity that people deficient in NK cells suffer from persistent viral infections. These functions of NK cells are important in regards to understanding medicinal mushrooms and their role in antiviral immunity.

Imagine the immune response to a newly inhaled viral particle. This virus enters the healthy person’s lungs and invades the lung epithelium. Once an epithelial cell is infected, it releases Type 1 Interferon, which turns circulating NK cells into cytotoxic effector NK cells (NK cells primed to seek out and kill virally infected cells). The cytotoxic effector NK cells promptly start the process of seeking out infected cells and the innate immune response commences. At the same time, there are resident macrophages in the respiratory epithelium and throughout the body. These macrophages (“big eaters”), are also major players in the innate immune response. Their role is to consume these virus particles and produce chemicals (cytokines and chemokines) that attract and engage more NK cells and also T cells. An important cytokine engaged in this process is interleukin-12 (IL-12), which stimulates NK cells to not become cytotoxic, but rather effector NK cells. Unlike cytotoxic effector NK cells, these effector NK cells stimulate an inflammatory response via Type 2 Interferon (IFN-γ), at the site of infection. This inflammatory cascade consists of IL-1, IL-6, TNF-α, and IL-12, and is essential for viral eradication. It is only when this response is out of control that it becomes problematic and detrimental to the host.1,2,3,4 If this initial response is not primed for viral combat, viral particles continue to proliferate and infect more healthy cells. It is in this phase that medicinal mushrooms can have a great impact to prevent viral infections from taking over the host.

Cytokine Source Role
IFN-α Virus infected epithelium Circulating NK cells –>Cytotoxic effector NK cells
IL-12 Macrophage post virus consumption NK cells –> effector NK cells
IFN- γ Effector NK cells Macrophages –> inflammatory cytokines (TNF-α, IL-2, IL-1, IL-6)
TNF- α Macrophages Induces blood vessels more permeable, enabling effector cells to enter infected tissue
IL-1 Macrophages Induces blood vessels more permeable, enabling effector cells to enter infected tissue
IL-6 Macrophages Induces fat and muscle cells to metabolize, make heat and raise temperature of infected tissue
IL-12 Macrophages Recruits and activates NK cells –> secrete more cytokines that strengthen macrophage response to infection
IL-10 Toxic substances secreted by macrophages–> TH2 Suppress macrophage activation
IL-4 Toxic substances secreted by macrophages–> TH2 Suppress macrophage activation


COVID-19 is an excellent example of these two main immune responses.5 The first stage of infection is the less severe incubation phase. The previously mentioned immune response is imperative to eliminate the virus and keep disease from progressing to later, more severe stages. It is in the incubation stage that immune-stimulating therapies are most indicated. In more severe stages, the protective immune response is impaired and the virus will spread and destroy healthy cells. Because damaged cells induce inflammation, immune stimulation is less indicated and it is more favorable to treat with anti-inflammatory therapies. It is at this stage of disease, characterized by severe lung inflammation, that life-threatening respiratory disorders occur and the feared cytokine storm is initiated. The cytokine storm is an influx of inflammatory cytokines. It is an overdramatic immune response that is harmful to the host and can often lead to acute respiratory distress syndrome. The inflammatory cytokines that are so important for stage one of the disease ( IL-1, IL6, TNF-α, and IL-12) are now abundant, destructive and out of control.6,7

The two phase division of the immune response is very important . The first immune response is protective and a response that can be altered through diet and lifestyle: our base response when initially combating infection. As mentioned previously, this is the phase where medicinal mushrooms are most indicated. They are primers of the first response to viral particles.


Antiviral immunity in healthy adults

The most informative studies exploring the interaction of medicinal mushrooms and the immune response are done on healthy human adults. In these studies it is ideal to see cytokine and NK cell levels before and after mushroom intake in healthy people to get a good idea of how exactly the mushrooms are priming our innate response. There are not many studies of this kind, but there are a few.

Healthy Korean men who took 1.5g/day of powdered extract of Cordyceps militaris brown rice culture for four weeks had their blood analyzed before and after treatment. Levels of NK cells, IFN-y and IL-12 were examined in blood samples before and four weeks after therapy began. There was a significant increase in NK cells and IFN-y and no difference in IL-12.8 Cordyceps sinensis mycelium extract, in combination with the endoparasitic fungus that commonly exists with C. sinensis, Paecilomyces hepialid, also had immune-stimulating activity in healthy adults. In this study, people were given 1.43g/day and after eight weeks the cytotoxic activity of NK cells was significantly higher than at baseline (before therapy.)9 Wild fruiting bodies of Cordyceps species are incredibly expensive and are therefore rarely, if ever used in research. However, It is likely that cultivated fruiting bodies have similar medicinal qualities.10,11,12

Another study with 52 healthy males and females aged 21-41 consumed either 5g or 10g of Lentinus edodes (shiitake mushroom) daily for four weeks. Eating the Shiitake for four weeks led to increased proliferation of NK cells and IgA, decreased CRP (a marker of inflammation), and an increase in IL-4, Il-10, TNF-α and IL-1. Serving size had no impact on cytokine levels, except that two servings of Shiitake increased serum IL-4.13 Shiitake is a good example of the dynamic effects that some mushrooms have on the immune system. Shiitake both increased inflammatory cytokines (IL-1, TNF-α) and anti-inflammatory cytokines (IL-10 and IL-4) simultaneously, illustrating the use of the term immunomodulatory: it is neither a pure stimulator nor a depressor of the immune system. This may mean that immune modulating mushrooms are safe and effective to take during both phases of the viral immune response, and in fact, may have inhibitory effects during the cytokine storm of acute respiratory distress syndrome.

Grifola frondosa (Maitake) also exhibits this modulating activity.  G. frondosa fruiting body extract produced both immune stimulatory IL-2, TNF-α and IFN-γ and suppressive IL-10 in breast cancer survivors taking 5-7g/kg per day of mushrooms extract for three weeks.14

The combination formula of Trametes versicolor(Turkey tail) and Salvia miltiorrhiza IMG_7864(Red sage root, or Dan Shen) given at 50mg/kg and 20mg/kg respectively for four months showed significant immunomodulatory effects in healthy adults. There was a significant increase in PBMC (peripheral blood mononuclear cells – NK, B and T cells) gene expression of IL-2 receptor, increase in T helper cells and the ratio of T helper cells to cytotoxic T cells. There is also a significant increase in IFN-γ.15 There is little information in western herbal and mycological medicine about the use of plant and mushroom combination formulas. Dan Shen is known to ‘move the qi of the blood’ and in combination with the immune stimulating activity of Turkey tail, has promise as a very useful combination for immune therapy.

Not all fungi are created equally as immune modulators. When the β-glucan isolate, lentinax, from L. edodes mycelium was administered to healthy adults, there was no difference seen in NK cells and inflammatory cytokines between treatment and control groups.16 This juxtaposes the previous Shiitake study where the subjects consumed whole mushrooms and did have immune stimulatory effects. Contrary to what has been suggested in in vitro research 17,18 a mushroom that showed no benefit in vivo was Agaricus blazeii. Healthy elderly women consumed 300mg of A. blazeii fruiting body extract three times daily for 60 days, and there was no change in levels of IFN-γ, IL-6 and TNF-α after administration.19 Perhaps the dose was too low in this study, further research is needed.

Mushroom Cytokine response Immune response (simplified) Phase of viral response most applicable
Grifola frondosa (Maitake) IL-10

IL-2, TNF-α, IFN- γ



Possibly severe phase

Prevention/incubation phase

Lentinus edodes (Shitake) IL-4, IL-10

TNF-α, IL-1



Possibly severe phase

Prevention/incubation phase

Cordyceps spp. IFN- γ Pro-inflammatory Prevention/incubation
Trametes versicolor (Turkey tail) with Salvia miltiorrhiza (Red sage) IL-2, IFN- γ Pro-inflammatory Prevention/incubation 

Mushrooms as immune-modulators

The increases seen in IL-10 and IL-4 after Maitake, Shiitake, and Cordyceps mycelium intake are important as they relate to TH2 immune responses. TH2 responses are anti-parasitic and anti-allergic, but through the lens of viral immunity and inflammation, are anti-inflammatory. In fact, IL-10 is considered an anti-inflammatory master regulator. 20,21,22,23 IL-10 is essential for defending the host from tissue destruction during acute phases of immune responses, though it is not as desirable in the initial phase of infection, where a higher TH1 (inflammatory) response is required. At this stage, IL-10 can downregulate antigen presentation in macrophages and dendritic cells and can lead to chronic infection.24 During the later stages of infection, however IL-10 levels can determine host survival and higher concentrations of IL-10 have been associated with better outcomes in patients with acute respiratory distress syndrome.25Cancer Cell Interactions

This is immune modulation. As depicted, mushrooms are neither solely inflammatory, nor anti-inflammatory, and so should be utilized as such. The safety of medicinal mushroom use at different phases of the immune response is debatable. It is most likely that if mushrooms and mushroom extracts are consumed as preventative medicine, and the immune response is primed, the host won’t succumb to infection in the first place. There is some concern that if IL-10 is too high during the initial phase, the infection will become chronic, but since the mushrooms are simultaneously stimulating inflammatory cytokines, this isn’t likely.

  TH1 TH2
Associated cytokines IL-2, IL-12, IFN-γ, TNF-α IL-4, IL-10, IL-5, IL-6, IL-13, IL-9


Mushroom Derived β-glucans and the Immune Response

The most studied immune-stimulating constituents derived from medicinal mushrooms are 1,3/1,6-β-glucans. β-glucans, or polysaccharides, are present in all mushrooms as they are an integral component of the mushroom cell wall. Macrophages in the gut have specific receptors for β-glucans, most significantly Dectin-1, complement receptor 3 and TLR-2.26,27 When β-glucans bind to these receptors, an immune response is initiated. Because of this, in most studies, polysaccharides have been deemed the ‘active’ constituents in regards to immune activation.  Therefore, isolation and administration of these compounds is most commonly seen in the literature.

Pleuran, a polysaccharide derived from Oyster mushrooms

There are a number of human trials exploring the use of pleuran, a polysaccharide extracted from Pleurotus ostreatus (Oyster mushroom) as a therapeutic agent in respiratory infections. As respiratory infections are most commonly of viral origin,28 it seems appropriate to discuss this research here. In a double-blinded, placebo-controlled, randomized multicentric study, 175 children treated with pleuran  experienced a significant reduction in the frequency of recurrent respiratory tract infections.29 These findings agreed with a Spanish study investigating 166 children aged one to ten years old who were also treated with pleuran for recurrent respiratory infection.30

Advantageous respiratory effects of pleuran are also observed in adult athletes. A study IMG_6690of 50 athletes treated with pleuran over a three month period found a significant reduction in the frequency of upper respiratory tract infections compared to athletes treated with placebo. Blood samples of the athletes showed significantly higher levels of circulating NK cells in the pleuran group as compared to the placebo group.31

Oyster mushrooms contain almost 33% polysaccharides,32 so we can deduce from these studies that consuming whole Oyster mushrooms and/or Oyster mushroom aqueous extracts could be beneficial for the prevention of respiratory infections.

Immune-stimulating polysaccharides in late stage cancer patients

The polysaccharides isolated from Ganoderma lucidum, also known as ‘Ganopoly’, were administered at 1800mg three times daily in late stage cancer patients, and there was a significant increase in NK cells, IL-1, IL-6 and IFN-γ after administration.33 Another isolate, polysaccharide krestin (PSK), is a protein-bound polysaccharide derived and isolated from Trametes versicolor. In a phase one clinical trial, breast cancer patients consumed 6 or 9g of PSK for six weeks, leading to an increase in CD8 T cells and NK cells.34 Another isolated polysaccharide, Maitake D fraction (derived from the fruiting body of G. frondosa), was administered to patients with stage 2-4 cancer aged 46-84 at doses between 40 and 150mg twice daily. IL-2 and NK cell activity was detected through peripheral blood draw and both were significantly increased after administration35 The research on isolated, mushroom-derived polysaccharides was intended to prove anti-cancer activity, but because of the close similarities of anti-cancer and antiviral immunity,36 it also suggests that polysaccharides support antiviral immunity in late stage cancer patients.

In vivo healthy animal trials

Polysaccharides from G. lucidum, L. edodes and G. frondosa administered to healthy mice significantly increase macrophage phagocytosis and NK cell activity.37 Other studies have demonstrated similar immune-enhancing effects on healthy mice with G. frondosa and L. edodes extracts exhibiting increases in IL-12, IL-6, and IFN-γ. In this study, the combination of the G. frondosa and L. edodes extracts have a stronger effect than either extract alone.38 Maitake D fraction increases IL-12 and IFN-γ in healthy mice along with a significant decrease in IL-4 and IL-10.39 C. militaris extract also increases IL-12 and TNF-α cell activity in H1N1 infected mice.40

In vivo animal cancer models

A number of in vivo animal trials explore different mushroom extracts with similar immune effects. Many of these animal studies are cancer models, so they will be mentioned briefly. Agaricus hot water extracts increase IFN-γ, IL-6 and IL-1,41,42 Reishi polysaccharide and triterpene extracts increase inflammatory NFkB, TNF- α, IL1-b, IL-2, IFN-γ 43,44,45,46 Maitake extract increases IL-12, IFN-γ and TNF-α, IL-147,48 and PSK increases IL-12.49 Ganoderma polysaccharides increase NK cells and cytotoxic T cells, IL-1, IL-6 and IL-1.50,51

Because these are cancer models, so it is not completely clear if the same effects would take place in healthy animal models, though we can deduct from other experiments using healthy volunteers and healthy animals that it is likely that similar immune effects would occur.

In vitro antiviral activity

There are a number of fungal constituents that have antiviral activity in-vitro, including polysaccharides, indole alkaloids, terpenoids, polyketides and lignan derivatives.  Agaricus subfruescens and Grifola frondosa act directly on viral particles, β-glucan protein from A. subfruescens inhibits viral adsorption into the cell,  polysaccharides from A. subfruescens and polysaccharopeptide from T. versicolor inhibit viral replication, and triterpenes from Ganoderma spp directly kill virus proteins.52

The fruiting body ethanol-water extract of T. versicolor extract increases the TH1-related cytokines IL-2, IL-12, IL-18 and IFN-γ.53,54 As most of the research done on T. versicolor is with an isolated constituent, PSK, it is significant that this study, which used whole fruiting body extract, exhibits immune stimulating qualities.

Maitake fruiting body extract does not show direct antiviral activity to influenza A but does exhibit antiviral activity through macrophage activation and an increase in TNF-α production. 55

L. edodes mycelium directly inhibits influenza virus growth at early phases of infection, possibly during the entry process of viral particles to host cells. The in vivo administration stimulated an increase in innate immunity as well, suggesting that Shiitake mycelium has antiviral effects through both inhibition of initial viral replication and immune stimulation.56

A little known mushroom, Cryptoporus volvatus, the Cryptic Globe Fungus, has shown cryptanti-viral activity through multiple mechanisms. Aqueous extracts of the fruiting body inhibit porcine respiratory syndrome virus infection by repressing viral entry, viral RNA expression, possibly viral protein synthesis, cell-to-cell spread, and the release of virus particles from the host cell. C. volvatus also inhibits influenza virus replication in vitro and in vivo.57,58,59

The aqueous extract of Phellinus igniarius, or Fire Sponge, shows virucidal activity against influenza A and B viruses, including 2009 pandemic H1N1, human H3N2, avian H9N2, and oseltamivir-resistant H1N1 viruses. The study concludes that this extract may interfere with one or more early events in the viral replication cycle, including viral attachment to the target cell. 60

In vitro research is what propels in vivo research forward, but it is important to take this information with a grain of salt and understand this is what may happen in the human body, and not necessarily what will happen.

Anti-neuraminidase activity

A highly valued antiviral action in pharmaceuticals is neuraminidase inhibition. This is the mechanism of the commonly known antivirals Tamilflu and Rilenza. Neuraminidase is found on the surface of influenza viruses and allows viruses to be released from the host cell so they can then infect other healthy human cells. Neuraminidase inhibitors have been shown to improve the outcome of patients with leukemia and influenza. 61 Medicinal mushrooms and their constituents have been shown to have neuraminidase phimg_7902inhibitory activity in vitro and in vivo. Ganoderic acids, triterpenes found in Ganoderma species, have broad spectrum inhibition against influenza neuraminidases, specifically H5N1 andH1N1.62,63 Both the fruiting body and mycelial extracts of Phellinus spp. have neuraminidase inhibiting actions as well. 64,65,66  While there is still more research needed in this area, it is possible that Reishi and Phellinus species could be beneficial in treating viruses that utilize neuraminidase.

This paper focuses on mushrooms as antiviral therapies for enveloped, influenza-like viruses, but there is in vitro evidence to suggest medicinal mushrooms have antiviral activity towards many different viruses.67,68 Ganoderma lucidum has shown to be active against enterovirus, 69 human papillomavirus (HPV),70,71 herpes simplex virus (HSV)72,73,74,75 hepatitis B (HBV)76 and Epstein Barr virus (EBV).77 Cordyceps militaris has anti-hepatitis C (HCV) activity.78 Trametes versicolor is active against human immunodeficiency virus HIV.79.80 Grifola frondosa is active against HSV-181 and HBV.82 Inonotus obliquus has anti-HSV, 83,84 anti-HCV 85and anti-HIV86 activity and Lentinula edodes has anti-HBV 87 and anti-HSV 88 activity. Although not explored in this paper, these antiviral actions are interesting and worth considering for further dissection in future research.

Having a basic understanding of our complex immune system is important in understanding the role that mushrooms play as antivirals. As we have seen, mushrooms are immune modulators and can stimulate inflammatory and anti-inflammatory responses simultaneously. Mushrooms are most likely to be useful as preventative medicine before infection occurs, though if there is an initial infection, Cordyceps, Maitake, Shiitake, Turkey tail and Oyster mushroom may prevent infection from becoming more severe. If infection does become severe, the mushrooms that also stimulate IL-10 – Maitake, Cordyceps and Shiitake, could also be beneficial. In the wake of the current viral pandemic, these mushrooms should be further explored and utilized as medicine. Further research is essential in elucidating their potential effects.

antiviral mechanisms of medicinal mushrooms


Work cited

  1. “Innate Immunity: the Induced Response to Infection.” The Immune System, by Peter Parham, Garland Science, 2015, pp. 68–78.
  2. Jost S, Altfeld M. Control of Human Viral Infections by Natural Killer Cells. Annu Rev Immunol. 2013;31(1):163-194. doi:10.1146/annurev-immunol-032712-100001
  3. Vidal, Sylvia M. Khakoo, SalminI. Biron CA. Natural Killer Cell Responses during Viral Infections: Flexibility and Conditioning of Innate Immunity by Experience. 2011;1(6):497-512. doi:10.1016/j.coviro.2011.10.017.Natural
  4. Waggoner, Stephen N. Reighard, Seth D., Gyurova IE et al. Roles of natural killer cells in antiviral immunity. Curr Opin Virol. 2016;16:15-23. doi:10.1016/j.physbeh.2017.03.040
  5. Shi Y, Wang Y, Shao C, Huang J, Gan J, Huang X. COVID-19 infection : the perspectives on immune responses. Cell Death Differ. 2020. doi:10.1038/s41418-020-0530-3
  6. Liu CH, Kuo SW, Ko WJ, et al. Early measurement of IL-10 predicts the outcomes of patients with acute respiratory distress syndrome receiving extracorporeal membrane oxygenation. Sci Rep. 2017;7(1):1-10. doi:10.1038/s41598-017-01225-1
  7. Van Erp EA, Van Kampen MR, Van Kasteren PB, De Wit J. Viral infection of human natural killer cells. Viruses. 2019;11(3):1-13. doi:10.3390/v11030243
  8. Kang, Ho Joon, Baik, Hyun Wook KSJ et al. Cordyceps militaris Enhances Cell-Mediated Immunity in Healthy Korean Men. 2015;18(October 2014):1164-1172. doi:10.1089/jmf.2014.3350
  9. Jung SJ, Jung ES, Choi EK, Sin HS, Ha KC, Chae SW. Immunomodulatory effects of a mycelium extract of Cordyceps (Paecilomyces hepiali; CBG-CS-2): A randomized and double-blind clinical trial. BMC Complement Altern Med. 2019;19(1):1-8. doi:10.1186/s12906-019-2483-y
  10. Zhu L, Tang Q, Zhou S, et al. Isolation and purification of a polysaccharide from the caterpillar medicinal mushroom Cordyceps militaris (Ascomycetes) fruit bodies and its immunomodulation of RAW 264.7 macrophages. Int J Med Mushrooms. 2014;16(3):247–257. doi:10.1615/intjmedmushr.v16.i3.50
  11. Lee JS, Hong EK. Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris. Int Immunopharmacol. 2011;11(9):1226–1233. doi:10.1016/j.intimp.2011.04.001
  12. Wang M, Meng XY, Yang RL, et al. Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice. Carbohydr Polym. 2012;89(2):461–466. doi:10.1016/j.carbpol.2012.03.029
  13. Dai X, Stanilka JM, Rowe CA, et al. Consuming Lentinula edodes (Shiitake) Mushrooms Daily Improves Human Immunity: A Randomized Dietary Intervention in Healthy Young Adults. J Am Coll Nutr. 2015;34(6):478-487. doi:10.1080/07315724.2014.950391
  14. Deng G. A phase I/II trial of a polysaccharide extract from Grifola frondosa in breast cancer patients inmunological effects. J Cancer Res Clin Oncol. 2013;135(9):1215-1221. doi:10.1007/s00432-009-0562-z.A
  15. Wong CK, Tse PS, Wong ELY, Leung PC, Fung KP, Lam CWK. Immunomodulatory effects of Yun Zhi and Danshen capsules in health subjects – A randomized, double-blind, placebo-controlled, crossover study. Int Immunopharmacol. 2004;4(2):201-211. doi:10.1016/j.intimp.2003.12.003
  16. Gaullier J-M, Sleboda J, Øfjord ES, et al. Supplementation with a soluble β-glucan exported from Shiitake medicinal mushroom, Lentinus edodes (Berk.) singer mycelium: a crossover, placebo-controlled study in healthy elderly. Int J Med Mushrooms. 2011;13(4):319-326. Accessed June 10, 2019.
  17. Hetland G, Johnson E, Lyberg T, Kvalheim G. The mushroom Agaricus blazei murill elicits medicinal effects on tumor, infection, allergy, and inflammation through its modulation of innate immunity and amelioration of Th1/Th2 imbalance and inflammation. Adv Pharmacol Sci. 2011;2011(Figure 2). doi:10.1155/2011/157015
  18. Cui L, Sun Y, Xu H, Xu H, Cong H, Liu J. A polysaccharide isolated from Agaricus blazei Murill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant. Oncol Lett. 2013;6(4):1039-1044. doi:10.3892/ol.2013.1484
  19. Lima CUJO, Souza VC, Morita MC, Chiarello MD, Karnikowski MGDO. Agaricus blazei Murrill and Inflammatory Mediators in Elderly Women : A Randomized Clinical Trial. 2011:336-342. doi:10.1111/j.1365-3083.2011.02656.x
  20. O’Garra A, Vieira PL, Vieira P, Goldfeld AE. IL-10-producing and naturally occurring CD4+ Tregs: limiting collateral damage. J Clin Invest. 2004;114(10):1372–1378
  21. Couper KN, Blount DG, Riley EM. IL-10: the master regulator of immunity to infection. J Immunol. 2008;180(9):5771–5777. [PubMed] [Google Scholar]
  22. Hawrylowicz CM. Regulatory T cells and IL-10 in allergic inflammation. J Exp Med. 2005;202(11):1459–1463
  23. Wu K, Bi Y, Sun K, Wang C. IL-10-producing type 1 regulatory T cells and allergy. Cell Mol Immunol. 2007;4(4):269–275
  24. Rojas JM, Avia M, Martín V, Sevilla N. IL-10: A multifunctional cytokine in viral infections. J Immunol Res. 2017;2017. doi:10.1155/2017/6104054
  25. Liu CH, Kuo SW, Ko WJ, et al. Early measurement of IL-10 predicts the outcomes of patients with acute respiratory distress syndrome receiving extracorporeal membrane oxygenation. Sci Rep. 2017;7(1):1-10. doi:10.1038/s41598-017-01225-1
  26. Fang J, Wang Y, Lv X. Structure of a β -glucan from Grifola frondosa and its antitumor effect by activating Dectin-1 / Syk / NF- κ B signaling. 2012:365-377. doi:10.1007/s10719-012-9416-z
  27. Akramienė, Dalia, Kondrotas, Anatolijus Didžiapetrienė J et al. Effects of B-glucans on the immune system. Medicina (B Aires). 2007;43(8). doi:10.4271/911515
  28. Charlton CL, Babady E, Ginocchio CC, Hatchette TF, Jerris RC, Li Y, Loeffelholz M, McCarter YS, Miller MB, Novak-Weekley S, Schuetz AN, Tang Y-W, Widen R, Drews SJ. 2019. Practical guidance for clinical microbiology laboratories: viruses causing acute respiratory tract infections. Clin Microbiol Rev 32:e00042-18. .00042-18.
  29. Jesenak M, Hrubisko M, Majtan J, Rennerova Z, Banovcin P. Anti-allergic Effect of Pleuran ( b -glucan from Pleurotus ostreatus ) . Jesenak M, Urbanclkova I, Banovcin P. Respiratory Tract Infections and the Role of Biologically Active Polysaccharides in Their. Nutrients. 2017:1-12. doi:10.3390/nu9070779 in Children with Recurrent Respiratory Tract Infections. 2014;474(March 2013):471-474.
  30. Pico Sirvent L, Sapena Grau J, Morera Ingles M, Rivero Urgell M. Effect of supplementation with β–glucan from Pleurotus ostreatus in children with recurrent respiratory infections. Ann Nurr Metab. 2013; 63 (1): 1378
  31. Bergendiova K, Tibenska E. Pleuran ( b -glucan from Pleurotus ostreatus ) supplementation , cellular immune response and respiratory tract infections in athletes. 2011:2033-2040. doi:10.1007/s00421-011-1837-z.
  32. McCleary B V., Draga A. Measurement of β-Glucan in mushrooms and mycelial products. J AOAC Int. 2016;99(2):364-373. doi:10.5740/jaoacint.15-0289
  33. Gao Y, Tang W, Dai X, et al. Effects of water-soluble Ganoderma lucidum polysaccharides on the immune functions of patients with advanced lung cancer. J Med Food. 2005;8(2):159-168. doi:10.1089/jmf.2005.8.159
  34. Torkelson CJ, Sweet E, Martzen MR, et al. Phase 1 Clinical Trial of Trametes versicolor in Women with Breast Cancer. Int Sch Res Netw. 2012;2012. doi:10.5402/2012/251632
  35. Kodama N, Komuta K, Nanba H. Activation of NK Cells in Cancer Patients. 2003;6(4):371-377.
  36. Müller L, Aigner P, Stoiber D. Type I interferons and natural killer cell regulation in cancer. Front Immunol. 2017;8(MAR):1-11. doi:10.3389/fimmu.2017.00304
  37. Mb YY, Fu W, Fu M, He G, Traore L. The immune effects of edible fungus polysaccharides compounds in mice. 2007;16(Suppl l):258-260.
  38. Vetvicka V, Vetvickova J. Immune-enhancing effects of Maitake (Grifola frondosa) and Shiitake (Lentinula edodes) extracts. Ann Transl Med.2014;2(2):14. doi:10.3978/j.issn.2305-5839.2014.01.05
  39. Noriko Kodama Yukihito Murata Hiroaki Nanba. Administration of a Polysaccharide from Grifola frondosa Stimulates Immune Function of Normal Mice Noriko. 2004;7(2):141-145.
  40. Lee HH, Park H, Sung GH, et al. Anti-influenza effect of Cordyceps militaris through immunomodulation in a DBA/2 mouse model. J Microbiol. 2014;52(8):696-701. doi:10.1007/s12275-014-4300-0
  41. Takimoto H, Kato H, Kaneko M, Kumazawa Y. Amelioration of skewed Th1/ Th2 balance in tumor-bearing and asthma-induced mice by oral administra- tion of Agaricus blazei extracts. Immunopharmacol Immunotoxicol. 2008;30(4):747-760.
  42. Lin JG, Fan MJ, Tang NY, et al. An extract of Agaricus blazei Murill adminis- tered orally promotes immune responses in murine leukemia BALB/c mice in vivo. Integr Cancer Ther. 2012;11(1):29-36.
  43. Zhang S, Nie S, Huang D, Li W, Xie M. Immunomodulatory effect of Ganoderma atrum polysaccharide on CT26 tumor-bearing mice. Food Chem. 2013;136(3-4):1213-1219.
  44. Wang G, Zhao J, Liu J, Huang Y, Zhong JJ, Tang W. Enhancement of IL-2 and IFN-gamma expression and NK cells activity involved in the anti-tumor effect of ganoderic acid Me in vivo. Int Immunopharmacol. 2007;7(6):864- 870.
  45.  Wang PY, Zhu XL, Lin ZB. Antitumor and immunomodulatory effects of polysaccharides from broken-spore of Ganoderma lucidum. Front Pharmacol. July 2012;3:135.
  46. Wang G, Zhao J, Liu J, Huang Y, Zhong J-J, Tang W. Enhancement of IL-2 and IFN-γ expression and NK cells activity involved in the anti-tumor effect of ganoderic acid Me in vivo. Int Immunopharmacol. 2007;7(6):864-870. doi:10.1016/j.intimp.2007.02.006
  47. Kodama N, Mizuno S, Nanba H, Saito N. Potential antitumor activity of a low-molecular-weight protein fraction from Grifola frondosa through enhancement of cytokine production. J Med Food. 2010;13(1):20-30. doi:10.1089/jmf.2009.1029
  48. Masuda Y, Murata Y, Hayashi M, Nanba H. Inhibitory effect of MD-Fraction on tumor metastasis: involvement of NK cell activation and suppression of intercellular adhesion molecule (ICAM)-1 expression in lung vascular endothelial cells. Biol Pharm Bull. 2008;31(6):1104–1108. doi:10.1248/bpb.31.1104
  49. Lu H, Yang Y, Gad E, et al. TLR2 agonist PSK activates human NK cells and enhances the antitumor effect of HER2-targeted monoclonal antibody therapy. Clin Cancer Res. 2011;17(21):6742–6753. doi:10.1158/1078-0432.CCR-11-1142
  50. Zhu XL, Chen AF, Lin Z Bin. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. J Ethnopharmacol. 2007;111(2):219-226. doi:10.1016/j.jep.2006.11.013
  51. Wang C, Wang Y. Abstracts from the 2nd International Symposium on Phytochemicals in Medicine and Food (2-ISPMF). Chin Med. 2018;13(S1):1-63. doi:10.1186/s13020-018-0172-2
  52. Linnakoski R, Reshamwala D, Veteli P, Cortina-Escribano M, Vanhanen H, Marjomäki V. Antiviral agents from fungi: Diversity, mechanisms and potential applications. Front Microbiol. 2018;9(OCT). doi:10.3389/fmicb.2018.02325
  53. Ho CY, Lau CBS, Kim CF, et al. Differential effect of Coriolus versicolor (Yunzhi) extract on cytokine production by murine lymphocytes in vitro. Int Immunopharmacol. 2004;4(12):1549-1557. doi:10.1016/j.intimp.2004.07.021
  54. Jeong SC, Yang BK, Kim GN, et al. Macrophage-stimulating activity of polysaccharides extracted from fruiting bodies of Coriolus versicolor (Turkey tail mushroom). J Med Food. 2006;9(2):175-181. doi:10.1089/jmf.2006.9.175
  55. Obi N, Hayashi K, Miyahara T, et al. Inhibitory effect of TNF-α produced by macrophages stimulated with Grifola frondosa extract (ME) on the growth of influenza A/Aichi/2/68 Virus in MDCK cells. Am J Chin Med. 2008;36(6):1171-1183. doi:10.1142/S0192415X08006508
  56. Kuroki T, Lee S, Hirohama M, et al. Inhibition of Influenza Virus Infection by Lentinus edodes Mycelia Extract Through Its Direct Action and Immunopotentiating Activity. Front Microbiol. 2018;9:1164. doi:10.3389/fmicb.2018.01164
  57. Gao L, Sun Y, Si J, et al. Cryptoporus volvatus extract inhibits influenza virus replication in vitro and in vivo. PLoS One. 2014;9(12). doi:10.1371/journal.pone.0113604
  58. Gao L, Zhang W, Sun Y, et al. Cryptoporus volvatus Extract Inhibits Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) In Vitro and In Vivo. PLoS One. 2013;8(5). doi:10.1371/journal.pone.0063767
  59. Ma Z, Zhang W, Wang L, et al. A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. PLoS One. 2013;8(11). doi:10.1371/journal.pone.0079333
  60. Lee S, Kim JI, Heo J, et al. The anti-influenza virus effect of Phellinus igniarius extract. J Microbiol. 2013;51(5):676–681. doi:10.1007/s12275-013-3384-2
  61. Chemaly RF, Torres HA, Aguilera EA, et al. Neuraminidase Inhibitors Improve Outcome of Patients with Leukemia and Influenza: An Observational Study. Clin Infect Dis. 2007;44(7):964-967. doi:10.1086/512374
  62. Zhu Q, Bang TH, Ohnuki K, Sawai T, Sawai K, Shimizu K. Inhibition of neuraminidase by Ganoderma triterpenoids and implications for neuraminidase inhibitor design. Sci Rep. 2015;5(AUGUST):13194. doi:10.1038/srep13194
  63. Zhu T, Kim S-H, Chen C-Y. A Medicinal Mushroom: Phellinus Linteus. Curr Med Chem. 2008;15(13):1330-1335. doi:10.2174/092986708784534929
  64. Kim J, Kim D, Hwang BS, et al. Mycobiology Neuraminidase Inhibitors from the Fruiting Body of Phellinus igniarius. 2016:117-120.
  65. Yeom JH, Lee IK, Ki DW, Lee MS, Seok SJ, Yun BS. Neuraminidase inhibitors from the culture broth of Phellinus linteus. Mycobiology.2012;40(2):142-144. doi:10.5941/MYCO.2012.40.2.142
  66. Song AR, Sun XL, Kong C, et al. Discovery of a new sesquiterpenoid from Phellinus ignarius with antiviral activity against influenza virus. Arch Virol. 2014;159(4):753–760. doi:10.1007/s00705-013-1857-6
  67. Teplyakova T V. antiviral activity of polyporoid mushrooms (higher basidiomycetes) from Altai Mountains (Russia). 2012:37-45.
  68. Krupodorova T, Rybalko S, Barshteyn V. Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and herpes simplex virus type 2 in cell culture. Virol Sin. 2014;29(5):284-290. doi:10.1007/s12250-014-3486-y
  69. Zheng DS, Chen LS. Triterpenoids from Ganoderma lucidum inhibit the activation of EBV antigens as telomerase inhibitors. Exp Ther Med. 2017;14(4):3273-3278. doi:10.3892/etm.2017.4883
  70. Hernández-Márquez E, Lagunas-Martínez A, Bermudez-Morales VH, et al. Inhibitory activity of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (higher Basidiomycetes) on transformed cells by human papillomavirus. Int J Med Mushrooms. 2014;16(2):179–187. doi:10.1615/intjmedmushr.v16.i2.80
  71. Donatini B. Control of oral human papillomavirus (HPV) by medicinal mushrooms, Trametes versicolor and Ganoderma lucidum: a preliminary clinical trial. Int J Med Mushrooms. 2014;16(5):497–498. doi:10.1615/intjmedmushrooms.v16.i5.80
  72. Eo SK, Kim YS, Lee CK, Han SS. Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses. J Ethnopharmacol. 2000;72(3):475–481. doi:10.1016/s0378-8741(00)00266-x
  73. Oh KW, Lee CK, Kim YS, Eo SK, Han SS. Antiherpetic activities of acidic protein bound polysacchride isolated from Ganoderma lucidum alone and in combinations with acyclovir and vidarabine. J Ethnopharmacol. 2000;72(1-2):221–227. doi:10.1016/s0378-8741(00)00254-3
  74. Hijikata Y, Yamada S, Yasuhara A. Herbal mixtures containing the mushroom Ganoderma lucidum improve recovery time in patients with herpes genitalis and labialis. J Altern Complement Med. 2007;13(9):985–987. doi:10.1089/acm.2006.6297
  75. Eo SK, Kim YS, Lee CK, Han SS. Antiherpetic activities of various protein bound polysaccharides isolated from Ganoderma lucidum. J Ethnopharmacol. 1999;68(1-3):175–181. doi:10.1016/s0378-8741(99)00086-0
  76. Li YQ, Wang SF. Anti-hepatitis B activities of ganoderic acid from Ganoderma lucidum. Biotechnol Lett. 2006;28(11):837–841. doi:10.1007/s10529-006-9007-9
  77. Iwatsuki K, Akihisa T, Tokuda H, et al. Lucidenic acids P and Q, methyl lucidenate P, and other triterpenoids from the fungus Ganoderma lucidum and their inhibitory effects on Epstein-Barr virus activation. J Nat Prod. 2003;66(12):1582–1585. doi:10.1021/np0302293
  78. Ueda Y, Mori K, Satoh S, Dansako H, Ikeda M, Kato N. Anti-HCV activity of the Chinese medicinal fungus Cordyceps militaris. Biochem Biophys Res Commun. 2014;447(2):341–345. doi:10.1016/j.bbrc.2014.03.150
  79. Rodríguez-Valentín M, López S, Rivera M, Ríos-Olivares E, Cubano L, Boukli NM. Naturally Derived Anti-HIV Polysaccharide Peptide (PSP) Triggers a Toll-Like Receptor 4-Dependent Antiviral Immune Response. J Immunol Res. 2018;2018:8741698. Published 2018 Jul 15. doi:10.1155/2018/8741698
  80. Collins RA, Ng TB. Polysaccharopeptide from Coriolus versicolor has potential for use against human immunodeficiency virus type 1 infection. Life Sci. 1997;60(25):PL383–PL387. doi:10.1016/s0024-3205(97)00294-4
  81. Gu C, Li J, Chao F, Jin M, Wang X, Shen Z. Isolation , identification and function of a novel anti-HSV-1 protein from Grifola frondosa. 2007;75:250-257. doi:10.1016/j.antiviral.2007.03.011
  82. Gu CQ, Li J, Chao FH. Inhibition of hepatitis B virus by D-fraction from Grifola frondosa: synergistic effect of combination with interferon-alpha in HepG2 2.2.15 [published correction appears in Antiviral Res. 2007 Jul;75(1):91. Li, Jun-Wen [corrected to Li, JunWen]]. Antiviral Res. 2006;72(2):162–165. doi:10.1016/j.antiviral.2006.05.011
  83. Polkovnikova MV, Nosik NN, Garaev TM, Kondrashina NG, Finogenova MP, Shibnev VA. Vopr Virusol. 2014;59(2):45–48.
  84. Pan HH, Yu XT, Li T, et al. Aqueous extract from a Chaga medicinal mushroom, Inonotus obliquus (higher Basidiomycetes), prevents herpes simplex virus entry through inhibition of viral-induced membrane fusion. Int J Med Mushrooms. 2013;15(1):29–38. doi:10.1615/intjmedmushr.v15.i1.40
  85. Shibnev VA, Mishin DV, Garaev TM, Finogenova NP, Botikov AG, Deryabin PG. Antiviral activity of Inonotus obliquus fungus extract towards infection caused by hepatitis C virus in cell cultures. Bull Exp Biol Med. 2011;151(5):612–614. doi:10.1007/s10517-011-1395-8
  86. Shibnev VA, Garaev TM, Finogenova MP, Kalnina LB, Nosik DN. Vopr Virusol. 2015;60(2):35–38.
  87. Zhao YM, Yang JM, Liu YH, Zhao M, Wang J. Ultrasound assisted extraction of polysaccharides from Lentinus edodes and its anti-hepatitis B activity in vitro. Int J Biol Macromol. 2018;107(Pt B):2217–2223. doi:10.1016/j.ijbiomac.2017.10.100
  88. Santoyo,Susana, Ramirez-Anguiana Ana, christina Aldaris-Garcia L et al. Antiviral activities of Boletus edulis, Pleurotus ostreatus and Lentinus edodes extracts and polysaccharide fractions against Herpes simplex virus type 1. Phys Rev Lett. 2002;88(12):4. doi:10.1103/PhysRevLett.88.126404











Oxalates and Fungi

Cause for concern?

The most common manifestation of kidney stones is without a doubt, calcium oxalate stones – 80% of stones, to be exact. For this reason, people who are prone to stones tend to avoid high oxalate-containing foods. The most popular oxalate-rich delicacies: spinach, beets greens, chard, chocolate, tea, nuts, grains, and yes, some mushrooms. It is important to keep in mind that not all oxalate-rich foods will lead to kidney stones – chemistry is much more complex than that.

There was a recent report1 about an older Japanese woman with liver cancer who consumed 4-5 tsp of Chaga mushroom per day for 6 months, and happened to get nephrotoxicity. The postulated culprit: Chaga mushroom. Unfortunately, in this study, there was neither mention of the source of the Chaga, if this was the canker or mycelium, nor if the Chaga powder was an extract or not. This case report concluded that it must have been the high oxalate content in the Chaga mushroom that induced this nephrotoxicity.  Is this a true cause or simply a correlation? Should consumers be concerned about the oxalate content in in all Chaga products?

My hope is that this post will be educational, and readers will walk away with some answers to these questions.

This report got me thinking, about Chaga and oxalates and wondering, could this really be? I mean, they do look so similar. Could this be an example of the doctrine of signatures or is it possible that if you crush any golden substance into small enough pieces it may resemble calcium oxalate fragments? (No offense to the doctrine of signatures)

Some education about oxalates

First, not all oxalates are created equal. There are soluble and insoluble oxalates. Insoluble oxalates are already bound to minerals, for example: calcium oxalate, magnesium oxalate and iron oxalate. The insoluble oxalates in foods pass right into the GI and come out in feces. They will not be absorbed in the blood stream, and so they are not a concern for causing hyperoxaluria (high urine oxalates). Examples of soluble oxalates are potassium and sodium oxalate. Unlike soluble oxalates, these acids release free oxalate anions which can pass into the blood stream. Free oxalate can then bind with free calcium and make calcium oxalate crystals;

This distinction is important to keep in mind when considering high oxalate foods.

Mushrooms and Oxalates

White button mushroom and Shiitake mushroom are both very high in oxalates, but 99% of them are insoluble! So, don’t fret about these mushrooms – the oxalate will all come out in your shiit(ake).2

Screen Shot 2019-12-01 at 8.20.41 AM

Oyster mushrooms, on the other hand, are moderately high in oxalates, and 90% of them are soluble. They contain a comparable amount to chocolate, almonds, and grains.2 So if you can eat more oyster mushrooms than you can chocolate, you are my hero and you may want to eat them with some calcium containing foods – more about this later.

Pleurotus ostreatus

Lucky for kidney stone formers, they can continue to consume large quantities of Lion’s Mane (Hericium erinaceus), Cauliflower mushroom (Sparassis spp) and Reishi (Ganoderma spp). There are no soluble oxalates found in any of these mushrooms.2

Remember, for an organism to take the time and energy to make a molecule, the molecule must serve some purpose for that organism; oxalates are not made to cause kidney stones in humans. I hope to find more on this subject in relation to mushrooms, but for now, I found one study. According to this study, calcium oxalate crystals form in response to toxic metal stress.3 So perhaps mushrooms growing in environments where there are more toxic metals will in turn have a higher calcium oxalate load than those same species growing elsewhere.

Oxalates and Chaga

Oxalate samples were assessed in Chaga samples from Russia, Finland and Thailand. A hot water extract was made of the Chaga canker (the growth coming out of the tree, traditionally used as medicine) and oxalate content was assessed. Researchers found that for 1 gram of the Russian Chaga extract there was 97.6mg of soluble oxalic acid and 24mg of insoluble oxalic acid. The Finnish Chaga extract contained 55.62mg/g of soluble oxalates and 9.5mg/g of insoluble oxalates. Japan Food Research Laboratories measured oxalate content of the mushroom powder and concentrations of 11.2g total oxalate in 100g of powder. Another test from this laboratory on a separate Chaga sample was 2.8g/100g.1

Some perspective: These amounts are higher than most edible mushrooms, similar to the amount in almonds, peanuts, cereal grains and chocolate, and much lower than what is found in high oxalate foods like spinach, rhubarb and beet greens.4

Cooking and Oxalates

Oxalate load was compared in a 10g sample of dried mushrooms cooked versus not cooked. This study found that cooking slightly decreases oxalates, but also seems to increase them in certain cases. Interestingly, levels of soluble oxalate from forest harvested mushrooms was generally lower than cultivated mushrooms.2

Concerned about your mushroom intake and risk of kidney stones?

Here is some food for thought:

Calcium intake and oxalate absorption

People with low calcium diets can benefit from consuming calcium-containing foods along with oxalate-containing foods to decrease oxalate absorption. One study found that exogenous (dietary) calcium leads to a linear reduction of oxalate absorption within the range of daily intake of 200-1200mg of calcium.  This case has been documented in numerous studies5,6,7 and calcium supplements have been shown to lower the oxalate absorption. One study assessed this theory using 1000mg calcium supplement given simultaneously with soluble oxalates. They concluded that this technique will reduce oxalate absorption by 10%, however this is mostly effective if the person already has a low calcium diet.  When calcium is supplemented to an already high calcium diet, there is only a 1% decrease in oxalate absorption.


Perhaps this is an excellent reason to add milk to tea and coffee and of course, to your favorite mushroom drink. To give some perspective, 1 cup of dairy milk has about 305mg of calcium and 1 cup of fortified almond milk has about 300mg of calcium.

The microbiome and oxalate absorption

Within the microbiome resides a bacteria known as Oxalobacter formigenes.8  This superlative gut bacteria degrades oxalates and helps to prevent hyperoxaluria and kidney stones. So, for those concerned about the oxalate content in the mushrooms discussed, perhaps this little bacteria is your ally. Studies looking at oral intake of Oxalobacter  have shown less urinary oxalate excretion following administration of an oxalate heavy food load compared to when there was no Oxalobacter administered. Unfortunately, this bacteria is susceptible to antibiotic use. Specifically, clarithromycin and doxycycline have been shown to annihilate this little probiotic, leaving the human left behind to be more susceptible to kidney stones.


Representation of Oxalobacter formigenes activity. Utilizes oxalate as a source of energy through the oxalate-formate antiporter.9

This is all very interesting and something most people do not need to worry about. Though I would say, if you are an oxalate kidney stone former: don’t eat a ton of oyster mushrooms or drink multiple cups of Chaga tea per day. Do make sure to drink plenty of water and eat a diet with adequate amounts of calcium, and hope that antibiotic use hasn’t eradicated all of your Oxalobacter.


Work Cited

  1. Kikuchi Y, Seta K, Ogawa Y, et al. Chaga mushroom-induced oxalate nephropathy. Clin Nephrol. 2014;81(6):440-444. doi:10.5414/CN107655
  2. Savage GP, Nilzen V, Österberg K, Vanhanen L. Soluble and insoluble oxalate content of mushrooms. Int J Food Sci Nutr. 2002;53(4):293-296.doi:10.1080/09637480120057000
  3. Jarosz-Wilkolazka A, Gadd GM. Oxalate production by wood-rotting fungi growing in toxic metal-amended medium. Chemosphere. 2003;52(3):541-547. doi:10.1016/S0045-6535(03)00235-2
  4. Glamočlija J, Ćirić A, Nikolić M, et al. Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal “mushroom.” J Ethnopharmacol.2015;162:323-332. doi:10.1016/j.jep.2014.12.069
  5. Von Unruh GE, Voss S, Sauerbruch T, Hesse A. Dependence of oxalate absorption on the daily calcium intake. J Am Soc Nephrol. 2004;15(6):1567-1573.doi:10.1097/01.ASN.0000127864.26968.7F
  6. Bong WC, Vanhanen LP, Savage GP. Addition of calcium compounds to reduce soluble oxalate in a high oxalate food system. Food Chem. 2017;221:54-57. doi:10.1016/j.foodchem.2016.10.031
  7. Brogren M, Savage GP. Bioavailability of soluble oxalate from spinach eaten with and without milk products. Asia Pac J Clin Nutr. 2003;12(2):219-224.
  8. Duncan SH, Richardson AJ, Kaul P, Holmes RP, Allison MJ, Stewart CS. Oxalobacter formigenes and its potential role in human health. Appl Environ Microbiol.2002;68(8):3841-3847. doi:10.1128/AEM.68.8.3841-3847.2002
  9. Corica, Domenico & Romano, Claudio. (2015). Renal Involvement in Inflammatory Bowel Diseases. Journal of Crohn’s & colitis. 10. 10.1093/ecco-jcc/jjv138.
  10. Oxalates in Chaga – A Potential Health Threat By Michael W. Beug, Chair NAMA Toxicology Committee
  11. Mitchell T, Kumar P, Reddy T, et al. Dietary oxalate and kidney stone formation. Am J Physiol Renal Physiol. 2019;316(3):F409–F413. doi:10.1152/ajprenal.00373.2018

Lion’s Mane: a Psychosomatic Psychobiotic

I know you immediately think of the brain, but follow the vagus nerve from brain to gut and let’s just stay there for a while…


This mushroom is incredibly popular right now. Very hip. Very trendy.

Also of grand popularity are afflictions of the stomach, intestines and the colon. Many people have a visceral response to this overstimulating existence of modernity, and the most common viscera affected – the gut. Not only is the entire weight of the world living there, but the once-prolific community of microorganisms that once inhabited this space, and that would normally support the gut with the physiological burden that comes with this weight, has been significantly depleted. The reasons for this lack of microbial support are many, though we can just call it an unhealthy obsession with purity. The problem here is that this physiological burden that comes with the stress of the world, more often than not, becomes a pathology. Lion’s Mane extracts infiltrate the gut and the associated lymphoid tissue, regulating inflammation, decreasing oxidative damage, feeding the residing microbiota and modulating the immune system – all desired actions for healing, and hopefully avoiding, this potential pathology. Before being known as a nootropic medicinal, this mushroom was used in Traditional Chinese Medicine as a digestive tonic and to support gastrointestinal health. I have gone through the most current research to support the use of Lion’s Mane for gut health, and so overall human health.

Warning – most all of the research has been done on rats or mice. This is just the reality of most in vivo research and that is all we get until human clinical studies, which are incredibly difficult to find funding for – especially with natural products that don’t have potential to be synthesized into pharmaceuticals – can be done. So if you need to blame someone, blame capitalism and big pharma.

Rodent studies exploring Hericium Extracts and inflammatory bowel disease (IBD)

There have been many studies exploring variations of Lion’s Mane extracts and the effects of these extracts on rodents with chemically induced inflammatory bowel disease (I know it is fucked up). In one study1, mice were exposed to 2% dextran sulfate sodium (DSS) for 7 days to induce acute intestinal inflammation. These mice were then administered an ethanol extract of Hericium at a dose of 250mg/kg/day (This is a very big dose). Results showed that the treated mice had significant improvement in body weight, colon length and overall decreased intestinal bleeding compared with control mice. After administration of the extract, there was suppressed production of inflammatory mediators (TNF-alpha, IL-1â and IL-6) in colon tissue, as well as a decrease in oxidative damage potentiators (nitric oxide production, malondialdehyde, and superoxide dismutase). Overall, Lion’s Mane decreased inflammation and oxidative stress, leading to a decrease in mucosal damage and protection of mucosal epithelium.

Another in vivo study2 induced colitis in mice using this same DSS solution, and found similar results with Hericium polysaccharides.  In this study, researchers tested markers of inflammation and oxidative stress before and after administration of the polysaccharides. Similar to the previously described study, there was a marked improvement in clinical symptoms postulated to be due to the decrease in nitric oxide, malondialdehyde, superoxide dismutase and myeloperoxidase. There was also a significant reduction in inflammatory cytokines TNF alpha, IL-1ß, and IL-6. Other notable markers decreased after polysaccharide administration include COX-2 and iNOS. COX-2 is an important enzyme in the prostaglandin synthesis pathway and the target of NSAIDs (like aspirin). Reduction or inhibition of this enzyme leads to decreased pain and inflammation. Intriguingly, this extract also blocked phosphorylation of NF-kappaB, p56 and also reversed DSS- induced gut dysbiosis and maintained intestinal barrier integrity. NF-kappaB is the major transcription factor for inflammatory cytokine production and if it is not phosphorylated it will not be active, and so less overall inflammation. (I realize I am really driving home the idea that we want to reduce inflammation, and inflammation is NOT always bad, but in a condition like inflammatory bowel disease, where the inflammation is causing significant damage to the mucosa and destroying a person’s quality of life, inflammation is not desirable).

Another method of inducing colitis in rats is with a trinitrobenzene sulfonic acid (TNBS) enema. To further explore the therapeutic potential of various Hericium extracts on inflammatory bowel disease, another study3 had to be done, using rats with this TNBS induced colitis. After the TNBS enema, the rats exhibited symptoms of reduced activity, lethargy, weight loss, ruffled fur, and bloody stool. After 14 days of Lion’s Mane extract treatments, there was significant improvement in all symptoms. Serum levels of inflammation were also monitored during this time, and decreased significantly after treatment. The proportion of FOXP3 and IL-10 positive cells significantly increased, especially in the rats receiving the alcoholic extracts compared with the model group. FOXP3 is the transcription factor for T-regulatory cells, and T regulatory cells secrete IL-10, so increased levels indicate improved immune regulation.

Prebiotic activity: Another study4 using an IBD rat model explored immunomodulatory activity of the water soluble fungal protein, HEP3, extracted from the mushroom fruiting body. The mechanism proposed: regulation of the gut microbiota. The study explained that HEP3 is a protein, and its digestion and absorption is reliant on proteases and peptidases extracted from bacteria. The nitrogenous degradation products of protein digestion also serve as important nutrient and energy sources for some anaerobic organisms. Knowing this, HEP3 can significantly influence the diversity, structures, and metabolism of organisms and microorganisms. Evidence suggests that the diversity of gut microbiota is reduced in IBD patients, so finding treatments that can target both inflammation and increase biodiversity is ideal. In this study, after HEP3 administration, Bifidobacterium abundance increased significantly and the colon tissue damage, inflammation, other prebiotics and diversity and structures improved significantly. Bifidobacterium is a beneficial genus of bacteria, associated with enhanced gut health and overall human health. This study concluded that HEP3 improves the immune system via regulation of the structure and metabolism of gut microbiota. The researchers postulate that through this prebiotic role, HEP3 activates the proliferation and differentiation of T cells and stimulates antigen presenting cells.

This prebiotic activity was also found in a hot water extract of the fruiting body.5 The preparation method was to simmer dried and ground mushroom in water (1:10, w:v) for 8 hours followed by pouring ETOH over the extract and leaving overnight. The alcohol was used to precipitate the polysaccharides which were then collected and used as the treatment in question. This method of precipitating polysaccharides out with alcohol is typically how polysaccharides are extracted in a laboratory setting, and so when people say that alcohol will destroy the polysaccharides in your mushroom extract, that is simply not true. This study found that Lactobacillus plantarum was the probiotic most affected by the Hericium polysaccharides. At first, after administration of the polysaccharides, the bacterial population rapidly increased after 6h. Also, the molecular weight of the polysaccharide decreased due to the loss of glycosidic bonds from gastrointestinal digestion, leading researchers to suppose that digestion is vital for the bioactivity of these polysaccharides in the GI system.

 Immune modulation: Not only are these polysaccharides food for our gut bacteria, but they are also the primary constituent involved with immune modulation. There are many receptors in the phagocytes associated with mucosal immunity and the most often discussed regarding mushroom 𝛃-glucans is Dectin-1. Interestingly, one study6 found evidence that that the major pattern recognition receptors for Hericium polysaccharides are TLR2 and mannose receptor rather than Dectin-1. (fangfang wu 2017) In an in vivo mouse study assessing Hericium polysaccharides and their effect on cell mediated immunity (T-cells and phagocytes), humoral immunity (B-cells, complement and antimicrobial peptides), phagocytic capacity of peritoneal cavity phagocytes, and NK cell activity, results showed that Hericium polysaccharides improve immune function by functional enhancement of all of the above.


Neuroimmunity: mucosal immunity and vagus nerve regulation

The vagus nerve, the 10th cranial nerve and the queen of the parasympathetic response. This is the rest and digest, the chill and feel, the relax and pass response. The Vagus nerve also innervates the majority of our digestive organs, hence the ‘digest’ and ‘pass’ parts of the aforementioned idioms. Therefore, the mucosal immunity described previously, is also innervated by this nerve. Studies show that when vagus is dysfunctional, there is more GI inflammation and an overall dysregulated mucosal immunity.7,8 There is evidence for stress-induced alterations in gut flora and an associated increase in inflammatory markers in the gastrointestinal tract. Does it go both ways? If there is less stress, then there is less GI inflammation, but if there is more regulation of gut inflammation, increased intestinal integrity and a more diverse microbiome, will there be a more regulated stress response? Are we stimulating vagus by stimulating mucosal immunity, therefore eliciting a parasympathetic response? Is this actually the mechanism by which Lion’s Mane decreases depression and anxiety in post-menopausal women?9

Bidirectional gut-brain communication: One route of this communication is thought to begin through sensory information from the GI tract, and subsequent activation of neural, hormonal, and immunological signals. These signals can independently or cooperatively relay information to the central nervous system (CNS).10,11,12 There are a number of studies described in this review regarding increased probiotic intake associated with increased mood and less anxiousness. Specifically, probiotic supplementation with Lactobacillus helveticus and Bifidobacterium longum showed less self-reported negative mood and decreased urinary cortisol.13 A similar effect was also observed in healthy participants who consumed a mixture of Bifidobacterium bifidum and Bifidobacterium lactis, and Lactobacillus acidophilus, Brevibacillus brevis, Brevibacterium casei, Bifidobacterium salivarius, and Lactococcus lactis 14 Allen et al.15 found that healthy individuals fed Bifidobacterium longum had attenuated levels of cortisol and reduced subjective anxiety in response to the socially evaluated cold stress pressor test.  It does indeed seem to go both ways: decreasing inflammation and regulating the gut microbiome reduces anxiety and stress, and reduced anxiety and stress decreases inflammation and regulates the gut microbiome.

Lion’s Mane mushroom is more than one constituent that increases nerve growth factor synthase (the myopia of this mushroom’s medicine). Lion’s Mane, like all things living, is made up of many synergistic compounds that work together to keep this mushroom living and producing. These compounds also happen to play a major role in human health. This field of neuroimmunology is growing and the physiological effects of Lion’s Mane are an excellent example of the mechanisms of bidirectional gut-brain communication. Lion’s Mane is, in fact, the ultimate psychosomatic medicine, in the true meaning of the word – relating to the interaction of mind and body, the psyche and the soma, and a true psychobiotic.

*Psychobiotic is actually a word, I did not invent it. It is a medicine that affects the psyche by regulating the gut microbiome.


Disclaimer: Information from this post is not meant to diagnose or treat any disease.



Work Cited

  1. Qin M, Geng Y, Lu Z, Xu H, Shi JS, Xu X, Xu ZH. Anti-Inflammatory Effects of Ethanol Extract of Lion’s Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), in Mice with Ulcerative Colitis. Int J Med Mushrooms. 2016;18(3):227-34. doi: 10.1615/IntJMedMushrooms.v18.i3.50. PubMed PMID: 27481156.
  2. Ren Y, Geng Y, Du Y, et al. Polysaccharide of Hericium erinaceus attenuates colitis in C57BL/6 mice via regulation of oxidative stress, inflammation-related signaling pathways and modulating the composition of the gut microbiota. J Nutr Biochem. 2018;57:67-76. doi:10.1016/j.jnutbio.2018.03.005
  3. Diling C, Xin Y, Chaoqun Z, et al. Extracts from Hericium erinaceus relieve inflammatory bowel disease by regulating immunity and gut microbiota. Oncotarget. 2017;8(49):85838-85857. doi:10.18632/oncotarget.20689
  4. Diling C, Chaoqun Z, Jian Y, et al. Immunomodulatory activities of a fungal protein extracted from Hericium erinaceus through regulating the gut microbiota. Front Immunol. 2017;8(JUN). doi:10.3389/fimmu.2017.00666
  5. Yang Y, Zhao C, Diao M, et al. The Prebiotic Activity of Simulated Gastric and Intestinal Digesta of Polysaccharides from the Hericium erinaceus. Molecules. 2018;23(12):1-14. doi:10.3390/molecules23123158
  6. Sheng X, Yan J, Meng Y, Kang Y, Han Z, Tai G, Zhou Y, Cheng H. Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology. Food Funct. 2017 Mar 22;8(3):1020-1027. doi:10.1039/c7fo00071e. PubMed PMID: 28266682.
  7. Shea-Donohue T, Urban JF Jr. Neuroimmune Modulation of Gut Function. Handb Exp Pharmacol. 2017;239:247-267. doi: 10.1007/164_2016_109. Review. PubMed PMID: 28035531.
  8. Bailey MT. Psychological Stress, Immunity, and the Effects on Indigenous Microflora. Adv Exp Med Biol. 2016;874:225-46. doi: 10.1007/978-3-319-20215-0_11. Review. PubMed PMID: 26589222.
  9. Nagano M, Shimizu K, Kondo R, Hayashi C, Sato D, Kitagawa K, Ohnuki K. Reduction of depression and anxiety by 4 weeks Hericium erinaceus intake. Biomed Res. 2010 Aug;31(4):231-7. PubMed PMID: 20834180.
  10. Lach G, Schellekens H, Dinan TG, Cryan JF. Anxiety, Depression, and the Microbiome: A Role for Gut Peptides. Neurotherapeutics. 2018;15(1):36-59. doi:10.1007/s13311-017-0585-0
  11. Matteoli G, Boeckxstaens GE. The vagal innervation of the gut and immune homeostasis. Gut. 2013;62(8):1214-1222. doi:10.1136/gutjnl-2012-302550
  12. Fonseca RC, Bassi GS, Brito CC, et al. Vagus nerve regulates the phagocytic and secretory activity of resident macrophages in the liver. Brain Behav Immun. 2019;81(December 2018):444-454. doi:10.1016/j.bbi.2019.06.041
  13. Messaoudi M, Violle N, Bisson J-F, Desor D, Javelot H, Rougeot C. Beneficial psychological effects of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) in healthy human volunteers. Gut Microbes 2011;2:256– 261.
  14. Steenbergen L, Sellaro R, van Hemert S, Bosch JA, Colzato LS. A randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood. Brain Behav Immun 2015;48:258–264.
  15. Allen AP, HutchW, Borre YE, et al. Bifidobacterium longum 1714 as a translational psychobiotic: modulation of stress, electrophysiology and neurocognition in healthy volunteers. Transl Psychiatry 2016;6:e939.











Fall Transitions

Fall. The quintessential season of the mushroom. I very outwardly and not so secretly love when summer comes to an end. The giant ball of fire in the sky, reflecting off of the far too many reflective surfaces throughout the city like laser beams in a diamond shop, is finally going to be shrouded by clouds. What a treat. Although a creature of habit, I have a deep love for transition – seasons of transition, symbols of transition, momentous occasions initiating transition, etc. Perhaps this is why I love Fall, and perhaps this is why I have a tattoo of an ouroboros on my right arm, and most definitely why I love mushrooms. As the rains come and wash away the summer, this can be a time of deep introspection and with that, inevitable discomfort. Shorter days and a generally darker existence is not desirable to most people, but can be especially valuable for the psyche. So rather than seeking herbs or mushrooms to bring more ‘light’ during this time, how about seeking out plant and mushroom allies to support and encourage transition. I think of deciduous trees during this time, trees that innately hold the energy of transformation – the ability to move through seasons in different forms and continue to grow stronger each year. The tannins in the leaves of the Birch, Willow, Poplar and Aspen oxidize from green to brilliant yellow, red, and brown. They stand bare through winter – vulnerable, nude, yet with tremendous stature and strength. Imaginably, this is the medicine that these trees share, and the energy that we can cultivate. Look for patterns in nature to understand the medicine in nature.  Is it grace through transition that you desire? Look closer at the natural world that surrounds you. This grace encompasses you already.